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Fig. 2 Impact of postnatal age on serum and CSF levels of <t>IGF-1,</t> and activation of <t>IGF-1R</t> in the ChP of the immature brain. A-D. Levels of IGF-1 in serum (A) and CSF (B) in preterm rabbit pups, evaluated 5 h after s.c. injections of rhIGF-1/rhIGFBP-3 (8 mg/kg) or the corresponding vehicle solution at 0 (5), 24 (29) or 72 (77) hours postnatal age (corresponding time-point for termination within parentheses). The ratio of CSF IGF-1/serum IGF-1 was calculated (C). Linear model displaying the relation between the IGF-1 levels in serum and CSF in 0 (5) hours postnatal age pups (corresponding time-point for termination within parentheses) (D). Dark grey area represents the 95% confidence level. Data are presented as means ± SD (N = 6–9). Differences in IGF-1 concentrations were analyzed using one-way ANOVA with post hoc Tukey test for multiple comparisons of means. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. E-F. Western blot analysis of pERK1/2 and pPKB in ChP from preterm rabbit pups 5 h after s.c. administration of rhIGF-1/rhIGFBP-3 (8 mg/kg) at 0 (5, upper), 24 (29, middle) or 72 h (77, lower) postnatal age (corresponding time-point for termination within parentheses). Data from 3 different experiments are presented as means ± SD (N = 3–4). A representative blot is shown. Differences between groups were analyzed using Student’s t-test. *P ≤ 0.05; NS, not significant

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1) Product Images from "Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3."

Article Title: Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3.

Journal: Fluids and barriers of the CNS

doi: 10.1186/s12987-023-00460-1

Fig. 2 Impact of postnatal age on serum and CSF levels of IGF-1, and activation of IGF-1R in the ChP of the immature brain. A-D. Levels of IGF-1 in serum (A) and CSF (B) in preterm rabbit pups, evaluated 5 h after s.c. injections of rhIGF-1/rhIGFBP-3 (8 mg/kg) or the corresponding vehicle solution at 0 (5), 24 (29) or 72 (77) hours postnatal age (corresponding time-point for termination within parentheses). The ratio of CSF IGF-1/serum IGF-1 was calculated (C). Linear model displaying the relation between the IGF-1 levels in serum and CSF in 0 (5) hours postnatal age pups (corresponding time-point for termination within parentheses) (D). Dark grey area represents the 95% confidence level. Data are presented as means ± SD (N = 6–9). Differences in IGF-1 concentrations were analyzed using one-way ANOVA with post hoc Tukey test for multiple comparisons of means. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. E-F. Western blot analysis of pERK1/2 and pPKB in ChP from preterm rabbit pups 5 h after s.c. administration of rhIGF-1/rhIGFBP-3 (8 mg/kg) at 0 (5, upper), 24 (29, middle) or 72 h (77, lower) postnatal age (corresponding time-point for termination within parentheses). Data from 3 different experiments are presented as means ± SD (N = 3–4). A representative blot is shown. Differences between groups were analyzed using Student’s t-test. *P ≤ 0.05; NS, not significant

Figure Legend Snippet: Fig. 2 Impact of postnatal age on serum and CSF levels of IGF-1, and activation of IGF-1R in the ChP of the immature brain. A-D. Levels of IGF-1 in serum (A) and CSF (B) in preterm rabbit pups, evaluated 5 h after s.c. injections of rhIGF-1/rhIGFBP-3 (8 mg/kg) or the corresponding vehicle solution at 0 (5), 24 (29) or 72 (77) hours postnatal age (corresponding time-point for termination within parentheses). The ratio of CSF IGF-1/serum IGF-1 was calculated (C). Linear model displaying the relation between the IGF-1 levels in serum and CSF in 0 (5) hours postnatal age pups (corresponding time-point for termination within parentheses) (D). Dark grey area represents the 95% confidence level. Data are presented as means ± SD (N = 6–9). Differences in IGF-1 concentrations were analyzed using one-way ANOVA with post hoc Tukey test for multiple comparisons of means. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. E-F. Western blot analysis of pERK1/2 and pPKB in ChP from preterm rabbit pups 5 h after s.c. administration of rhIGF-1/rhIGFBP-3 (8 mg/kg) at 0 (5, upper), 24 (29, middle) or 72 h (77, lower) postnatal age (corresponding time-point for termination within parentheses). Data from 3 different experiments are presented as means ± SD (N = 3–4). A representative blot is shown. Differences between groups were analyzed using Student’s t-test. *P ≤ 0.05; NS, not significant

Techniques Used: Activation Assay, Western Blot

Fig. 4 Angiogenesis-related gene expression and IGF-1R activation in ChPE cells upon stimulation with rhIGF-1/rhIGFBP-3 or rhIGF-1. A. Outline of experimental in vitro setup. B. Western blot analysis of pERK1/2 and pPKB in primary murine ChPE cells following exposure to rhIGF-1/rhIGFBP-3 (300 ng/ml), rhIGF-1 (60 ng/ml) or cell culture media only (Control) for 15 min. Data from are presented as means ± SD (N = 4, pooled samples). C. Heat map, showing respective group mean absolute fold change and hierarchical clustering (N = 3–4), comparing gene expression of 84 genes related to angio genesis using RT2 Profiler PCR Array in primary murine ChPE cells upon stimulation with rhIGF-1/rhIGBP-3 (300 ng/ml), rhIGF-1 (60 ng/ml) or culture medium only (Control) for 2 h. D - I. Individual analysis of selected target genes from the RT2 Profiler PCR Array, Bai1 (D), nitric oxide synthase 3 (Nos3) (E), plasminogen (Plg) (F), C-C motif chemokine 11 (Ccl11) (G), chemokine (C-X-C motif) ligand 2 (Cxcl2) (H) and epidermal growth factor (Egf) (I). Data are presented as means ± SD (N = 4). Differences in gene expression were analyzed using one-way ANOVA with post hoc Tukey for multiple comparisons of means, *P ≤ 0.05. P3-P8, postnatal day 3–8

Figure Legend Snippet: Fig. 4 Angiogenesis-related gene expression and IGF-1R activation in ChPE cells upon stimulation with rhIGF-1/rhIGFBP-3 or rhIGF-1. A. Outline of experimental in vitro setup. B. Western blot analysis of pERK1/2 and pPKB in primary murine ChPE cells following exposure to rhIGF-1/rhIGFBP-3 (300 ng/ml), rhIGF-1 (60 ng/ml) or cell culture media only (Control) for 15 min. Data from are presented as means ± SD (N = 4, pooled samples). C. Heat map, showing respective group mean absolute fold change and hierarchical clustering (N = 3–4), comparing gene expression of 84 genes related to angio genesis using RT2 Profiler PCR Array in primary murine ChPE cells upon stimulation with rhIGF-1/rhIGBP-3 (300 ng/ml), rhIGF-1 (60 ng/ml) or culture medium only (Control) for 2 h. D - I. Individual analysis of selected target genes from the RT2 Profiler PCR Array, Bai1 (D), nitric oxide synthase 3 (Nos3) (E), plasminogen (Plg) (F), C-C motif chemokine 11 (Ccl11) (G), chemokine (C-X-C motif) ligand 2 (Cxcl2) (H) and epidermal growth factor (Egf) (I). Data are presented as means ± SD (N = 4). Differences in gene expression were analyzed using one-way ANOVA with post hoc Tukey for multiple comparisons of means, *P ≤ 0.05. P3-P8, postnatal day 3–8

Techniques Used: Gene Expression, Activation Assay, In Vitro, Western Blot, Cell Culture, Control

Fig. 6 Schematic illustration of the main findings in the present study. Subcutaneous administration of rhIGF-1/rhIGBP-3 to preterm rabbits (E29), results in a higher level of IGF-1 in serum and CSF at postnatal age at administration of 0 h compared to 24 and 72 h. This is in turn correlated with an increased IGF-1R activation in the ChP at a postnatal age of 5 h, and expression of genes involved in the ChP development. Stimulating ChPE cells with rhIGF-1/ rhIGFBP-3 or rhIGF-1 results in an activation the IGF-1R and an increase in protein synthesis involved in ATP production and mRNA processing

Figure Legend Snippet: Fig. 6 Schematic illustration of the main findings in the present study. Subcutaneous administration of rhIGF-1/rhIGBP-3 to preterm rabbits (E29), results in a higher level of IGF-1 in serum and CSF at postnatal age at administration of 0 h compared to 24 and 72 h. This is in turn correlated with an increased IGF-1R activation in the ChP at a postnatal age of 5 h, and expression of genes involved in the ChP development. Stimulating ChPE cells with rhIGF-1/ rhIGFBP-3 or rhIGF-1 results in an activation the IGF-1R and an increase in protein synthesis involved in ATP production and mRNA processing

Techniques Used: Activation Assay, Expressing

Incubation:

Article Title: Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3.
Article Snippet: .. Sections were then incubated with a cocktail of primary antibodies against IGF-1R (goat polyclonal IgG, 2 μg/ml, diluted in PBS-TX-BSA, AF-305-NA, R&D System, McKinley Place, MN, USA) and IGF-1 (mouse monoclonal IgG, 2 μg/ml, diluted in PBS-TX-BSA, AM33345PU-S, Origene, Herford, Germany) for 16 h at 4 °C. .. Following rinses in PBS-TX, sections were incubated for 45 min at room temperature with a mixture of AF488 conjugated secondary antibodies against goat IgG (IGF-1R) and RhodamineRX-conjugated antibodies against mouse IgG (IGF-1, both from Jackson ImmunoResearch, West Grove, PA, USA, diluted 1:200 in PBS-TX).

Article Title: Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3
Article Snippet: .. Sections were then incubated with a cocktail of primary antibodies against IGF-1R (goat polyclonal IgG, 2 μg/ml, diluted in PBS-TX-BSA, AF-305-NA, R&D System, McKinley Place, MN, USA) and IGF-1 (mouse monoclonal IgG, 2 μg/ml, diluted in PBS-TX-BSA, AM33345PU-S, Origene, Herford, Germany) for 16 h at 4 °C. .. Following rinses in PBS-TX, sections were incubated for 45 min at room temperature with a mixture of AF488 conjugated secondary antibodies against goat IgG (IGF-1R) and RhodamineRX-conjugated antibodies against mouse IgG (IGF-1, both from Jackson ImmunoResearch, West Grove, PA, USA, diluted 1:200 in PBS-TX).

Image Search Results

The IGF-1R signaling pathway plays a primary role in the neurogenesis effect of β -cyc. ( A , B ) Effects of the IGF-1R inhibitor T9576 and PI3K/AKT inhibitor LY294002 on the neurite outgrowth elicited by β -cyc, its combination with low-dose NGF, and NGF 1 ng/mL. ( C ) Western blot analysis and digitalization of IGF-1R and p-IGF-1R levels induced by β -cyc dose-dependently. ( D ) Western blot analysis and digitalized results of p-IGF-1R, IGF-1R, p-PI3K, PI3K, p-AKT, and AKT induced by β -cyc in a time-dependent experiment. Each experiment was repeated three times. The data were expressed as a mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with the negative control; ### p < 0.001, compared with the 3 µM β -cyc group; $$$ p < 0.001, compared with the combination group of β -cyc with low-dose NGF; && p < 0.01, compared with the low-dose NGF, ns indicates no significant difference, compared with the low-dose NGF.

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β -Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells

doi: 10.3390/ijms25189763

Figure Lengend Snippet: The IGF-1R signaling pathway plays a primary role in the neurogenesis effect of β -cyc. ( A , B ) Effects of the IGF-1R inhibitor T9576 and PI3K/AKT inhibitor LY294002 on the neurite outgrowth elicited by β -cyc, its combination with low-dose NGF, and NGF 1 ng/mL. ( C ) Western blot analysis and digitalization of IGF-1R and p-IGF-1R levels induced by β -cyc dose-dependently. ( D ) Western blot analysis and digitalized results of p-IGF-1R, IGF-1R, p-PI3K, PI3K, p-AKT, and AKT induced by β -cyc in a time-dependent experiment. Each experiment was repeated three times. The data were expressed as a mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with the negative control; ### p < 0.001, compared with the 3 µM β -cyc group; $$$ p < 0.001, compared with the combination group of β -cyc with low-dose NGF; && p < 0.01, compared with the low-dose NGF, ns indicates no significant difference, compared with the low-dose NGF.

Article Snippet: The antibodies against IGF-1R (CAT No.: 3027S), phospho-IGF-1R (Tyr1131, CAT No.: 3024S), PI3 kinase (CAT No.: 4249S), phospho-PI3 kinase (Tyr458, CAT No.: 4228S), AKT (CAT No.: 9272S), phospho-AKT (Ser473, CAT No.: 9271S), GR (CAT No.: 12041T), PLC (CAT No.: 14008S), phospho-PKC (CAT No.: 2261S), and PKC (CAT No.: 38168S) were obtained from Cell Signaling Technology, Boston, MA, USA, and the antibodies against phospho-GR (CAT No.: AF2004) and phospho-PLC γ (CAT No.: AF4454) were obtained from Affinity Biosciences, Cincinnati, OH, USA.

Techniques: Western Blot, Negative Control

Related inhibitors on the modulation of the IGF-1R/PI3K/AKT pathway induced by β -cyc in PC12 cells. ( A ) The phosphorylation of the IGF-1R, PI3K, and AKT induced by β -cyc or its combination with low-dose NGF were downregulated by T9576. ( B ) The phosphorylation of PI3K and AKT stimulated by β -cyc or its combination with low-dose NGF was reduced by LY294002. Each experiment was repeated three times. The data were expressed as a mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001, compared with the negative control; ### p < 0.001, compared with the 3 µM β -cyc group; $ p < 0.05 and $$$ p < 0.001, compared with the combination group of β -cyc with low-dose NGF.

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β -Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells

doi: 10.3390/ijms25189763

Figure Lengend Snippet: Related inhibitors on the modulation of the IGF-1R/PI3K/AKT pathway induced by β -cyc in PC12 cells. ( A ) The phosphorylation of the IGF-1R, PI3K, and AKT induced by β -cyc or its combination with low-dose NGF were downregulated by T9576. ( B ) The phosphorylation of PI3K and AKT stimulated by β -cyc or its combination with low-dose NGF was reduced by LY294002. Each experiment was repeated three times. The data were expressed as a mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001, compared with the negative control; ### p < 0.001, compared with the 3 µM β -cyc group; $ p < 0.05 and $$$ p < 0.001, compared with the combination group of β -cyc with low-dose NGF.

Techniques: Phospho-proteomics, Negative Control

Target prediction of β -cyc by CETSA. ( A , B ) The interaction between β -cyc and IGF-1R and GR protein was studied using CETSA. β -cyc at 3 and 1 μM were assessed to investigate the expression changes in IGF-1R and GR protein at varying temperatures by Western blotting. ( C ) Quantification of the data for panels ( A , B ). ( D , E ) The modified CETSA method was utilized to explore the interaction between β -cyc and the IGF-1R protein/GR protein following alterations in the concentration of β -cyc. ( F ) Quantitative analysis for panels D and E. Each experiment was repeated three times. The data were expressed as a mean ± SEM. *** p < 0.001, compared with the negative control.

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β -Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells

doi: 10.3390/ijms25189763

Figure Lengend Snippet: Target prediction of β -cyc by CETSA. ( A , B ) The interaction between β -cyc and IGF-1R and GR protein was studied using CETSA. β -cyc at 3 and 1 μM were assessed to investigate the expression changes in IGF-1R and GR protein at varying temperatures by Western blotting. ( C ) Quantification of the data for panels ( A , B ). ( D , E ) The modified CETSA method was utilized to explore the interaction between β -cyc and the IGF-1R protein/GR protein following alterations in the concentration of β -cyc. ( F ) Quantitative analysis for panels D and E. Each experiment was repeated three times. The data were expressed as a mean ± SEM. *** p < 0.001, compared with the negative control.

Techniques: Expressing, Western Blot, Modification, Concentration Assay, Negative Control

Target prediction of β -cyc by DARTS assay. ( A ) Western blot analysis of IGF-1R and ( B ) GR protein level after adding different concentrations of pronase E. ( C ) Quantification of the data obtained from panels ( A , B ). ( D ) Protein level of IGF-1R after adding different concentrations of β -cyc (0.1, 1, 3, 10 µM) by Western blotting. IGF-1R was normalized with β -actin. ( E ) Western blot analysis of GR protein level after adding different concentrations of β -cyc (0.01, 0.1, 1, 10 µM). GR was normalized with β -actin. ( F ) Quantification of the data obtained from panels D-E. Each experiment was conducted three times. $ p < 0.05, $$ p < 0.01 and $$$ p < 0.001 in comparison to the untreated group; *** p < 0.001 compared to the negative control without pronase E; # p < 0.05 and ### p < 0.001 compared with the negative control with pronase E.

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β -Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells

doi: 10.3390/ijms25189763

Figure Lengend Snippet: Target prediction of β -cyc by DARTS assay. ( A ) Western blot analysis of IGF-1R and ( B ) GR protein level after adding different concentrations of pronase E. ( C ) Quantification of the data obtained from panels ( A , B ). ( D ) Protein level of IGF-1R after adding different concentrations of β -cyc (0.1, 1, 3, 10 µM) by Western blotting. IGF-1R was normalized with β -actin. ( E ) Western blot analysis of GR protein level after adding different concentrations of β -cyc (0.01, 0.1, 1, 10 µM). GR was normalized with β -actin. ( F ) Quantification of the data obtained from panels D-E. Each experiment was conducted three times. $ p < 0.05, $$ p < 0.01 and $$$ p < 0.001 in comparison to the untreated group; *** p < 0.001 compared to the negative control without pronase E; # p < 0.05 and ### p < 0.001 compared with the negative control with pronase E.

Techniques: Western Blot, Comparison, Negative Control

Proposed mechanism of action for β -cyc. β -cyc promotes neurogenesis by targeting IGF-1R and GR to activate downstream PI3K/AKT and PLC/PKC signaling pathways. The up arrow represents an increase in protein phosphorylation level.

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β -Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells

doi: 10.3390/ijms25189763

Figure Lengend Snippet: Proposed mechanism of action for β -cyc. β -cyc promotes neurogenesis by targeting IGF-1R and GR to activate downstream PI3K/AKT and PLC/PKC signaling pathways. The up arrow represents an increase in protein phosphorylation level.

Techniques: Protein-Protein interactions, Phospho-proteomics

Journal: Fluids and barriers of the CNS

Article Title: Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3.

doi: 10.1186/s12987-023-00460-1

Figure Lengend Snippet: Fig. 2 Impact of postnatal age on serum and CSF levels of IGF-1, and activation of IGF-1R in the ChP of the immature brain. A-D. Levels of IGF-1 in serum (A) and CSF (B) in preterm rabbit pups, evaluated 5 h after s.c. injections of rhIGF-1/rhIGFBP-3 (8 mg/kg) or the corresponding vehicle solution at 0 (5), 24 (29) or 72 (77) hours postnatal age (corresponding time-point for termination within parentheses). The ratio of CSF IGF-1/serum IGF-1 was calculated (C). Linear model displaying the relation between the IGF-1 levels in serum and CSF in 0 (5) hours postnatal age pups (corresponding time-point for termination within parentheses) (D). Dark grey area represents the 95% confidence level. Data are presented as means ± SD (N = 6–9). Differences in IGF-1 concentrations were analyzed using one-way ANOVA with post hoc Tukey test for multiple comparisons of means. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. E-F. Western blot analysis of pERK1/2 and pPKB in ChP from preterm rabbit pups 5 h after s.c. administration of rhIGF-1/rhIGFBP-3 (8 mg/kg) at 0 (5, upper), 24 (29, middle) or 72 h (77, lower) postnatal age (corresponding time-point for termination within parentheses). Data from 3 different experiments are presented as means ± SD (N = 3–4). A representative blot is shown. Differences between groups were analyzed using Student’s t-test. *P ≤ 0.05; NS, not significant

Article Snippet: Sections were then incubated with a cocktail of primary antibodies against IGF-1R (goat polyclonal IgG, 2 μg/ml, diluted in PBS-TX-BSA, AF-305-NA, R&D System, McKinley Place, MN, USA) and IGF-1 (mouse monoclonal IgG, 2 μg/ml, diluted in PBS-TX-BSA, AM33345PU-S, Origene, Herford, Germany) for 16 h at 4 °C.

Techniques: Activation Assay, Western Blot

Journal: Fluids and barriers of the CNS

Article Title: Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3.

doi: 10.1186/s12987-023-00460-1

Figure Lengend Snippet: Fig. 4 Angiogenesis-related gene expression and IGF-1R activation in ChPE cells upon stimulation with rhIGF-1/rhIGFBP-3 or rhIGF-1. A. Outline of experimental in vitro setup. B. Western blot analysis of pERK1/2 and pPKB in primary murine ChPE cells following exposure to rhIGF-1/rhIGFBP-3 (300 ng/ml), rhIGF-1 (60 ng/ml) or cell culture media only (Control) for 15 min. Data from are presented as means ± SD (N = 4, pooled samples). C. Heat map, showing respective group mean absolute fold change and hierarchical clustering (N = 3–4), comparing gene expression of 84 genes related to angio genesis using RT2 Profiler PCR Array in primary murine ChPE cells upon stimulation with rhIGF-1/rhIGBP-3 (300 ng/ml), rhIGF-1 (60 ng/ml) or culture medium only (Control) for 2 h. D - I. Individual analysis of selected target genes from the RT2 Profiler PCR Array, Bai1 (D), nitric oxide synthase 3 (Nos3) (E), plasminogen (Plg) (F), C-C motif chemokine 11 (Ccl11) (G), chemokine (C-X-C motif) ligand 2 (Cxcl2) (H) and epidermal growth factor (Egf) (I). Data are presented as means ± SD (N = 4). Differences in gene expression were analyzed using one-way ANOVA with post hoc Tukey for multiple comparisons of means, *P ≤ 0.05. P3-P8, postnatal day 3–8

Techniques: Gene Expression, Activation Assay, In Vitro, Western Blot, Cell Culture, Control

Journal: Fluids and barriers of the CNS

Article Title: Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3.

doi: 10.1186/s12987-023-00460-1

Figure Lengend Snippet: Fig. 6 Schematic illustration of the main findings in the present study. Subcutaneous administration of rhIGF-1/rhIGBP-3 to preterm rabbits (E29), results in a higher level of IGF-1 in serum and CSF at postnatal age at administration of 0 h compared to 24 and 72 h. This is in turn correlated with an increased IGF-1R activation in the ChP at a postnatal age of 5 h, and expression of genes involved in the ChP development. Stimulating ChPE cells with rhIGF-1/ rhIGFBP-3 or rhIGF-1 results in an activation the IGF-1R and an increase in protein synthesis involved in ATP production and mRNA processing

Techniques: Activation Assay, Expressing

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